Skip to main content
. 2005 Jan 20;115(2):282–290. doi: 10.1172/JCI23394

Figure 11.

Figure 11

IL-1 induction of osteoclast precursor differentiation occurs within a broad temporal window. (A) WT marrow macrophages were cultured in the presence of M-CSF (50 ng/ml) and RANKL (10 ng/ml). IL-1 (1.25 ng/ml) or vehicle was added only once to each well. IL-1 addition occurred on sequential days from day 0 through day 4. TRAP stain was performed on day 5. (B) Osteoclast number in each culture condition described in A (#P < 0.0001 vs. no IL-1 addition). (C) WT marrow macrophages were cultured in the presence of M-CSF (50 ng/ml) plus RANKL (100 ng/ml). Total RNA was extracted with time and analyzed by RT-PCR for abundance of IL-1RI and IL-1RII mRNA.