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. 2017 Feb 15;13(5):854–867. doi: 10.1080/15548627.2017.1287653

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© 2017 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 1. — Effects of lysosomotropic and ionophore drugs on lysosome pH using LysoTracker. MCF10A cells were labeled with LysoTracker Red for 2 h in the presence of absence of bafilomycin A₁ (Baf, 100 nM), monensin (Mon, 50 μM), nigericin (Nig, 10 μM), chloroquine (CQ, 100 μM), hydroxychloroquine (HCQ, 100 μM), NH₄Cl (10 mM), betahistine (BH, 5 mM), procainamide (ProA, 5mM) or lidocaine hydrochloride (LH, 2.5 mM). (A) Quantification of LysoTracker fluorescence. Data are mean ± SD of 8 fields of view from 2 independent experiments. One-way ANOVA, P<0.0001. AU, arbitrary units. (B) Representative inverted grayscale images of LysoTracker staining in cells. Bar: 20 μm for all images.