Table 3.
Substrates of CHK1 and phosphorylation sites. These are manually validated results from RLIMS-P output. n/a indicates that the phosphorylated site is not described in the RLIMS-P output
| CHK1 Substrates | Site | PMIDs |
|---|---|---|
| AURKB | Ser-331 | 22024163, 23321637 |
|
| ||
| BLM | Ser-646 | 20719863 |
|
| ||
| BRCA2 | Thr-3387 | 24627786, 18317453 |
|
| ||
| CDC10 | n/a | 24006488 |
|
| ||
| CDC2 | Tyr-15 | 24996846, 11479224 |
|
| ||
| CDC25 | Ser-287 | 9744884 |
| Ser-99 | 10198041 | |
| n/a | 9923681, 11133168, 15272308, 9774107, 10469601, 17912454 | |
|
| ||
| CDC25A | Ser-123 | 12399544, 12759351 |
| Ser-178,Thr-507 | 14559997 | |
| Ser-73 | 12110582 | |
| Ser-75 | 12759351 | |
| Ser-76 | 14681206, 20348946, 18480045, 21252624, 20798862 | |
| Thr-504 | 15272308 | |
| n/a | 12110582, 12399544, 20609246, 18414041, 24022480, 19244340, 21851590, 15272308, 19638579, 23272087, 21347609, 9278511, 18480045 | |
|
| ||
| CDC25B | Ser-230,Ser-563 | 17003105 |
| n/a | 9278511, 10713667, 20798862 | |
|
| ||
| CDC25C | Ser-216 | 14681223, 9278511, 10676638, 11027648, 24922656, 15282313, 10557092, 22623962, 23874958, 20700484 |
| n/a | 18272544, 10090724, 9278511, 11479224, 11925443, 15220526, 10681541, 22941630, 20798862, 21347609, 11278490, 10068474, 24038466 | |
|
| ||
| CDK1 | n/a | 20798862 |
|
| ||
| CDKN1A | n/a | 21791608 |
|
| ||
| CDKN1C | n/a | 21791608 |
|
| ||
| Ser-296 | 23068608, 20053762, 21289283, 24996846 | |
| CHK1 | Ser-317,Ser-345 | 21851590 |
| n/a | 14681223, 15371427, 23548269, 23593009, 19421147 | |
|
| ||
| CK1D | n/a | 23861943 |
|
| ||
| CK2 | n/a | 15225637 |
|
| ||
| CLP1 | n/a | 22918952 |
|
| ||
| CLSPN | Thr-916 | 16963448 |
|
| ||
| Ser-80 | 22792081 | |
| CRB2 | Thr-73 | 22792081 |
|
| ||
| CSNK1D | Ser-328, Ser-331,Thr-397 | 23861943 |
| Ser-328,Ser-331,Ser-370,Thr-397 | 23861943 | |
| Ser-328,Thr-329,Ser-331,Ser-361,Ser-382 | 23861943 | |
|
| ||
| E2F6 | n/a | 23954429 |
|
| ||
| ENOS3 | Ser-1179 | 22001744 |
|
| ||
| ERRFI1 | Ser-251 | 22505024 |
|
| ||
| FANCD2 | n/a | 21926477 |
|
| ||
| FANCE | Thr-346,Ser-374 | 17296736 |
|
| ||
| H2AFX | Ser-345 | 24913641 |
|
| ||
| H2AX | Thr-16 | 20639511 |
|
| ||
| KAP1 | Ser-473 | 21851590 |
|
| ||
| LATS2 | Ser-408 | 21118956 |
| Ser-835 | 23886938 | |
|
| ||
| MAD2 | n/a | 23454898 |
|
| ||
| MDMX | Ser-367 | 16511572 |
|
| ||
| p33 (ING1b) | Ser-126 | 17585055 |
|
| ||
| p50 | n/a | 22152481 |
|
| ||
| PDS1 | n/a | 11390356, 17671432 |
|
| ||
| RAD51 | n/a | 18317453 |
|
| ||
| RAD9 | n/a | 24376897 |
|
| ||
| RASSF1 | Ser-184 | 24197116 |
|
| ||
| RB1 | n/a | 17380128 |
|
| ||
| RELA | Ser-612 | 15970704 |
| Thr-505 | 17962807 | |
|
| ||
| RPA1 | n/a | 16412704 |
|
| ||
| SETMAR | n/a | 25024738 |
| Ser-495 | 22231448 | |
|
| ||
| SYK | Ser-295 | 22585575 |
|
| ||
| TAU | n/a | 23550703 |
|
| ||
| TLK1 | n/a | 12660173 |
| Ser-695 | 24376897, 12955071 | |
|
| ||
| TP53 | Ser-20 | 15467443, 17339337 |
| Ser-23 | 23152407 | |
| n/a | 15659650, 11599922, 23272087 | |
|
| ||
| TP73 | Ser-47 | 14585975 |
|
| ||
| WEE1 | Ser-549 | 11251070 |