Fig 4. Antigen specific response in selected cultures from subject RM30.

Cell lines were established from T. cruzi specific cultures, based on results from experiment depicted on Fig 3. Cells were submitted to 2 PHA expansion cycles prior to specificity evaluation. Five thousand cells from each culture well were challenged with T. cruzi lysate, using 10⁴ autologous overnight-primed B-LCL per well as APC. SI was calculated for each well as the response against T. cruzi lysate divided by the cells baseline response (media only condition), cultures considered positive were those with an SI≥2 (dotted line). Bars show the mean values and standard deviation for three replicates of each measure.