Fig. 3. : LIVE/DEAD® viability of dengue virus (DENV)-2 infected Huh-7 cells treated with Uncaria guianensis bark (UGB) or leaf (UGL) extracts. Huh-7 hepatocyte cell line was infected with DENV-2 (TCID50 = 5 x 107/mL) for 24-72 h and viability was detected by the LIVE/DEAD® Fixable Dead Cell Stain Kit. Fluorescence intensity and percentage of labelled cells were determined using flow cytometry analysis (FL-4). Absolute cell counts were corrected according to the volume analysed. Six independent experiments with 3-4 replicates each are represented and were used for Kruskal-Wallis and Dunn’s multiple comparison tests. Significances: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.