Fig. 5. : non-structural protein NS1 detected in Huh-7 cell cultures after treatment with Uncaria guianensis bark (UGB) or leaf (UGL) extracts. Huh-7 hepatocyte cell line was infected with dengue virus (DENV)-2 (TCID50 = 5 x 107/mL) for 24-72 h and treated with different concentrations of UGB or UGL, dexamethasone (0.1 mM) or untreated. Non-structural protein NS1 was detected by enzyme-linked immunosorbent assay (ELISA) Dengue NS1 Ag kit Platelia in cell culture supernatants 24, 48 or 72 h after infection and treated UGL. The ratio represented was calculated the rate of the optical density (OD) obtained from samples and OD from NS 1 cut-off (average of duplicates from kit calibrator, CO). The values were represented as mean ± SEM. Three independent experiments with 1-3 replicates each are represented and were used for the Multiple t-test. * p < 0.05.