TABLE I. : Uncaria guianensis bark extract (UGB) and U. guianensis leaf extract (UGL) cytotoxicity in uninfected Huh-7 cells. Viability detection by MTT assay and comparison to control cells cultured without UG.
| UGB (µg/mL) | |||||
|---|---|---|---|---|---|
| Time after infection (h) | 100 | 50 | 25 | 10 | 1 |
| 24 | 78* | 97 | 98 | 100 | 99 |
| 48 | 87 | 93 | 98 | 102 | 106 |
| 72 | 83 | 94 | 96 | 98 | 99 |
| 96 | 76 | 79 | 76 | 78 | 84 |
|
| |||||
| UGL (µg/mL) | |||||
|
| |||||
| Time after infection (h) | 100 | 50 | 25 | 10 | 1 |
|
| |||||
| 24 | 69 | 73 | 79 | 93 | 93 |
| 48 | 72 | 77 | 78 | 90 | 92 |
| 72 | 55 | 70 | 78 | 86 | 93 |
| 96 | 46 | 60 | 64 | 66 | 67 |
*: cellular viability rates determined after Huh-7 cell line cultures were treated or not with UGB or UGL for 24-96 h. Percentages were normalised according to control optical density in MTT assay (GraphPad Prism 6) and means of replicates are represented. Four independent experiments were tested with 3-8 replicates each.