Figure 8. Sequence information for thrombospondin antibody and TSR peptide fragments used in this study.
(A) Alignment of the second TSR domains from human thrombospondin 1 and TSR proteins from the anemone Aiptasia pallida and the dinoflagellate Symbiodinium minutum. In red are the binding sites for glycosaminoglycans (GAGs) and CD36; greyscale indicates the % identity of the three sequences. Pink annotation indicates the TSR peptide sequence covering all three binding domains; inset are the synthetic peptide sequences for experimental peptides. In Peptide 2, the cysteine residues were replaced with alanine residues, as shown in red. (B) A section of the antibody-binding region of the human thrombospondin 1/2 antibody (H-300, sc-14013 from Santa Cruz Biotechnology), aligned to a TSR protein fragment from Aiptasia sp. Legends for Supplementary Material.