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Simulation error was calculated as the deviation of the YFP synthesis rate from the mean across an ensemble of 5000 identically simulations for each of the three kinetic models considered in the manuscript. The yfp mutant in the simulation had two CTA stall sites at Leu2 and Leu14 as shown in Figure 5. YFP synthesis rate was calculated relative to the no-stall control. Black line indicates a Gaussian fit.
