Fig. 3.

(A) Representative blot of extracellular signal-regulated kinase 5 (ERK5) activation in 2 hours of various flow conditions. Cells were serum starved for 2 hours prior to experimentation. There is activation of ERK5 in all flow conditions. (B) P-ERK5 standardized to actin. There are increased levels of P-ERK5 in all flow conditions except in static and 2-hour H2O₂ media for 2 hours. (C) Arbitrary densitometric measurements of ERK5 standardized to actin. There is decrease in ERK5 in all flow conditions. CONT; continuous laminar flow; PFF, pulsatile forward flow; SS, serum starvation; TFF, to-and-fro flow. * p < 0.05, ** p < 0.001.