Fig. 4.

(A) Representative blot of extracellular signal-regulated kinase 5 (ERK5) activation in 4 hours of various flow conditions. Cells were serum starved for 2 hours prior to experimentation. There is activation of ERK5 in nonpulsatile uniform flow and disturbed flow conditions. (B) Arbitrary densitometric measurements of P-ERK5 standardized to actin. There is activation of ERK5 in continuous laminar and disturbed flow conditions. (C) ERK5 standardized to actin showing decreased levels in all flow conditions. CONT; continuous laminar flow; PFF, pulsatile forward flow; SS, serum starvation; TFF, to-and-fro flow. * p < 0.05, ** p < 0.001.