(A) ERK5 activity in response to different types of flow. ERK5 levels significantly decreased in disturbed flow compared with continuous laminar flow, after 4 hours of stimulation both, continuous and disturbed flow decreased compared with pulsatile flow. (B) Activation of ERK5 increased in continuous and disturbed flow compared with pulsatile uniform flow. CONT, continuous laminar flow; PFF, pulsatile forward flow; TFF, to-and-fro flow. * p < 0.05, ANOVA analysis with post hoc test (Fischer's test).