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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Stem Cells. 2017 Mar 27;35(6):1493–1504. doi: 10.1002/stem.2606

Figure 3. Validation of the DOE model using spheroids predicted to have the greatest (Spheroid 1) and least (Spheroid 2) VEGF and PGE2 production.

Figure 3

Spheroid 1 (40,000 MSCs/spheroid, 1 μg/mL Pam3CSK4, 1% O2) versus Spheroid 2 (10,000 MSCs/spheroid, 0 μg/mL Pam3CSK4, 21% O2). (A) VEGF and (B) PGE2 were significantly increased in Spheroid 1 versus Spheroid 2 (n=4, **p<0.01 vs. Spheroid 2). (C) Stacked column chart of predominant anti-inflammatory cytokines, normalized to unconditioned media (α-MEM). Live (green)/dead (red) assay showing the viability of (D) Spheroid 1 and (E) Spheroid 2. Scale bar represents 500 μm; images captured using a 4X objective. (F) Spheroid size is dependent upon the number of cells per spheroid (n=4, ****p<0.0001 vs. Spheroid 2).