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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Stem Cells. 2017 Mar 27;35(6):1493–1504. doi: 10.1002/stem.2606

Figure 4. Macrophage phenotype is regulated by conditioned media (CM) from MSC spheroids.

Figure 4

(A) Spheroid 1 CM reduced mTNFα secretion by LPS-stimulated murine macrophages, while CM from Spheroid 2 and adherent MSCs treated in the same manner were less effective. (B) Spheroid 1 CM stimulated secretion of IL-10 by LPS-stimulated macrophages significantly better than CM from Spheroid 2 and adherent MSCs treated in the same manner. Significance is denoted by alphabetical letterings; groups with no significance are linked by the same letters, while groups with significance do not share the same letters (n=4). Abbreviations: Mϕ, macrophage; sMϕ, stimulated macrophage; PGE2, macrophages receiving 10 ng/mL PGE2; S1, Spheroid 1; S2, Spheroid 2; A1, Adherent 1, cultured with 1 μg/mL Pam3CSK4, under 1% O2; A2, Adherent 2, cultured with 0 μg/mL Pam3CSK4, under 21% O2.