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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Stem Cells. 2017 Mar 27;35(6):1493–1504. doi: 10.1002/stem.2606

Figure 5. CM from optimized MSC spheroids promotes capillary network formation.

Figure 5

Endothelial cell tubulogenesis in the presence of MSC-conditioned media measured by (A) the number of branch points per field of view and (B) total tubule length. Significance is denoted by alphabetical letterings; groups with no significance are linked by the same letters, while groups with significance do not share the same letters (n=4). Representative images of endothelial cell tubulogenesis on Matrigel in the presence of MSC-conditioned medium from (C) S1, (D) S2, and (E) EBM-2. Scale bar represents 500 μm; images captured using a 4X objective. Abbreviations: EGM-2, endothelial cell growth media, positive control; GF-Def EGM-2, growth factor deficient EGM-2, experimental control; EBM-2, endothelial basal media, negative control. S1, Spheroid 1; S2, Spheroid 2; A1, Adherent 1; A2, Adherent 2.