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. 2017 May 26;7:2433. doi: 10.1038/s41598-017-01150-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Whole Blood Device Operation. (a) Photomicrograph of: mid-Stage 1 showing large cluster deflected into product stream (white arrow), (b) exit of Stage 1, two large clusters exiting into Stage 1 product (white arrows), and (c) Stage 2 showing two small clusters being deflected out of blood stream (black arrows). Red blood cells are dark. Scale bar: 200 µm. (d) Image of Blood input vs. Waste, Stage 1 and Stage 2 outputs showing red blood cell content. (e) Log (10) depletion of red blood cells and white blood cells in Stage 1 (dark) and Stage 2 (light) outputs. Experiments conducted in triplicate, error bars represent one standard deviation.