Figure 2.

BRD4 Is a Negative Regulator of Autophagy Gene Expression

(A and B) KP-4 cells transfected with control or BRD4 siRNA were subjected to RNA-seq and gene ontology analyses (A) and RT-qPCR analysis (B).

(C) RT-qPCR analysis of KP-4 cells treated with DMSO, 500 nM JQ1, 500 nM I-BET151, or 500 nM OTX015 for 9 hr.

(D) RT-qPCR analysis of KP-4 cells treated with 500 nM JQ1 for the indicated time.

(E) RT-qPCR analysis of KP-4 cells overexpressing BRD4.

All data are shown as mean ± SD. In (A)–(D), n = 3 independent experiments; in (E), data are representative of two independent experiments performed in triplicate. ^∗p < 0.01, ^∗∗p < 0.05. See also Figure S2.