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. 2017 May 18;66(4):517–532.e9. doi: 10.1016/j.molcel.2017.04.027

Figure 4.

Figure 4

Starvation Leads to BRD4 Dissociation from Autophagy Gene Promoters

(A and B) KP-4 cells were starved for 4 hr followed by chromatin immunoprecipitation (ChIP) assay with BRD4 antibody (A) and RT-qPCR analysis (B).

(C) KP-4 cells infected with Cas9/hMOF sgRNA were subjected to ChIP assay with BRD4 antibody.

(D) KP-4 cells were starved for 4 hr followed by ChIP assay with H4K16Ac antibody.

(E and F) KP-4 cells infected with Cas9/SIRT1 sgRNA were starved for 4 hr followed by ChIP assay with BRD4 antibody (E) and RT-qPCR analysis (F). Western blot shows efficient SIRT1 depletion in Cas9/SIRT1 sgRNA-infected cells.

(G and H) KP-4 cells infected with Cas9/AMPKα1 and α2 sgRNAs were starved for 4 hr followed by immunoprecipitation with DBC1 antibody (G) and RT-qPCR analysis (H).

All data are shown as mean ± SD. In (A)–(F) and (H), n = 3 independent experiments. ∗p < 0.01, ∗∗p < 0.05, N.S., no significance. See also Figure S4.