Figure 5.

BRD4 Represses Autophagy Gene Expression through G9a

(A) Cell extracts from KP-4 cells were subjected to immunoprecipitation with G9a (upper) and BRD4 (lower) antibodies.

(B) KP-4 cells were starved for 4 hr followed by immunoprecipitation with BRD4 antibody.

(C–F) KP-4 cells were treated with 500 nM JQ1 for 9 hr (C and E). KP-4 cells harboring inducible control or BRD4 shRNA were treated with 500 ng/mL doxycycline (DOX) for 4 days (D and F). ChIP assays were performed using G9a (C and D) and H3K9diMe (E and F) antibodies.

(G and H) KP-4 cells infected with shRNA targeting G9a were transfected with BRD4 siRNA followed by RT-qPCR (G) and western blot (H).

(I) KP-4 cells overexpressing BRD4 were infected with shRNA targeting G9a.

All data are shown as mean ± SD. In (C)–(G), n = 3 independent experiments. ^∗p < 0.01, ^∗∗p < 0.05, N.S., no significance. See also Figure S5.