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. 2017 May 26;7:2442. doi: 10.1038/s41598-017-02766-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CV responses (a) and EIS (b) of the different modified electrodes in 0.1 M PBS containing 5 mM K₃[Fe(CN)₆]/K₄[Fe(CN)₆] containing 0.1 M KCl, respectively. Bare Au (curve a), electrode modified successively with Au/r-GO nanocomposites (curve b), mixture of capture anti-LC3B-II (Ab₁) and anti-Beclin-1 (Ab₁) (curve c),mixture of 10 ng/mL LC3B-II and Beclin-1 (curve d), mixture of anti-Beclin-1 (Ab₂)/Au NCs and anti-LC3B-II (Ab₂)/Au NCs (curve e). Effect of the incubation time (c) and pH (d) on the response of the immunosensor.