Table 1.

Viral RNAs sequenced from samples of A. bisporus fruitbodies and their length and homology to known viruses.

Name	Short name		Contig	Length	Closest virus	Family/Genus	e-value
Agaricus bisporus virus 2	AbV2		C23-C1	14566	† Cryphonectria hypovirus 2	Hypoviridae	7 × 10−145
Agaricus bisporus endornavirus 1	AbEV1		C40	12730	Rhizoctonia cerealis endornavirus 1	Endornaviridae	4 × 10−98
Agaricus bisporus virus 3	AbV3		C3	9340	Cherry green ring mottle virus	Betaflexiviridae	3 × 10−28
Agaricus bisporus virus 5	AbV5		C6	8371	Citrus leaf blotch virus	Betaflexiviridae	3 × 10−50
Agaricus bisporus virus 6	AbV6	RNA 1	C2	8848	Cherry mottle leaf virus	Betaflexiviridae	3 × 10−30
		RNA 2	C12	3559	‡ Odontoglossum ringspot virus	Virgaviridae	1 × 10−28
Agaricus bisporus virus 7	AbV7		C4	8759	Botrytis virus F	Gammaflexiviridae	6 × 10−36
Agaricus bisporus spherical virus	AbSV		C5	8540	Cherry rusty mottle associated virus	Betaflexiviridae	5 × 10−31
Agaricus bisporus virus 8	AbV8		C8	8280	Beet necrotic yellow vein virus	Benyvirus	2 × 10−21
Agaricus bisporus virus 9	AbV9		C13-C18-C14	7713	Botrytis virus F	Gammaflexiviridae	1 × 10−27
Agaricus bisporus virus 10	AbV10		C7	7033	Fusarium graminearum dsRNA mycovirus-1	1Unassigned	0
Agaricus bisporus virus 11	AbV11		C10	6981	Fusarium graminearum dsRNA mycovirus-1	1Unassigned	0
Agaricus bisporus virus 12	AbV12		C9	6893	Oyster mushroom spherical virus	2Unassigned	2 × 10−41
Agaricus bisporus virus 13	AbV13		C15	6202	Rice stripe necrosis virus	Benyvirus	7 × 10−41
Mushroom bacilliform virus	MBV		C11	4009	Mushroom bacilliform virus	Barnaviridae	0
Agaricus bisporus mitovirus 1	AbMV1		C41	3439	Mitovirus AEF-2013	Mitovirus	3 × 10−65
Agaricus bisporus virus 14	AbV14		C21	3482	Epirus cherry virus	Ourmiavirus	2 × 10−3
Agaricus bisporus virus 15	AbV15		C21a	3405	Cassava virus C	Ourmiavirus	5 × 10−3
Agaricus bisporus virus 16	AbV16	RNA 1	C22	1826	‡ Broad bean mottle virus	Bromoviridae	6 × 10−81
		RNA 2	C20	1949	‡ Hydrangea chlorotic mottle virus	Betaflexiviridae	9 × 10−27
		RNA 3	C29	781
		RNA 4	C33	558

The Name column corresponds to the proposed or given name for the discovered viruses. The Short Name column corresponds to a shortened version of the full name. The Contig column corresponds to contiguous RNA sequences assembled from the Illumina reads for each virus. The Length column is in RNA bases and corresponds to the total length of the virus. The Closest Virus column corresponds to the highest scoring homology (lowest e-value) to the given virus as identified with BLASTX. The e-value column indicates the likelihood of the BLASTX alignment occurring due to random chance. The Family/Genus column corresponds to the homologous viruses’ assigned taxonomic group.

¹Proposed new members of the Hypoviridae family.

²Proposed new member of the Tymovirales order.

^†The closest match was to a partial sequence of Agaricus bisporus virus X Acc.: CAD19173.

^‡Alignments found using DELTA-BLAST.