Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 May 26;7:2446. doi: 10.1038/s41598-017-02719-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Assessment of column IgG binding capacity and cross-linking efficiency. Serum protein electrophoresis (SPE) of native rabbit serum showing IgG levels (a). Column run-through following D84 anti-native BP rabbit serum loading on SpinTrap Column (b). Boxes indicate the gamma peak, containing IgG along with fibronectin and C-reactive protein. Gamma peak was reduced from 0.3 mg/dL in native serum to below 0.1 mg/dL in the run-through (n = 1). Calculated efficiency of rabbit IgG capture and cross-linking to the column determined by ELISA analysis of IgG content in native serum and column run-through (c). No difference in IgG capture or cross-linking efficiency was found between D0 versus D84 serum using a paired two-tailed Student’s t-test (n = 6 per timepoint, p = 0.4663, values represent the mean ± s.d.).