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. 2017 May 26;7:2446. doi: 10.1038/s41598-017-02719-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of antigenic protein identifications depending on type of extraction solution, SDS-H, NDSB-H, and NDSB-L. Venn diagram showing the number of antigenic proteins identified using each protein extraction methods (a). Pie charts demonstrating subcellular location of proteins depending on the protein extraction method used (b–d). Numbers associated with subcellular locations indicate number of antigens identified within that subcellular location. (n = 6 per extraction method).