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Effect of dosages of the Hex fraction on WT1 protein expression and total cell number in Molt4 cells. Molt4 cells were treated with various doses (0.5, 1.0, 1.5, and 2.0 μg/ml) for 48 h. (a) The levels of WT1 expression after treatment were evaluated by Western blotting; GAPDH was used as a loading control. (b) The total cell numbers were determined by the trypan blue dye exclusion method. * denotes a significant difference from the control group (p < 0.05).
