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. 2017 May 1;7(5):1068–1083.

Figure 3.

Figure 3

The effect of Flot2 knockdown on migration, invasion and proliferation of HCC cells. A. Wound healing assay shown that closing of the wound was much slower in MHCC97H-shFlot2 group than the control group. B, C. Compared with the control group, the invasive ability of Flot2 silenced group was significantly decreased by Transwell matrix penetration assay (MHCC97H-shFlot2 group: 48.3 ± 4.1 vs 102.8 ± 5.8, P < 0.01; SMMC-7721-shFlot2 group: 37.3 ± 3.9 vs 105.5 ± 5.1, P < 0.01). D. The CCK8 assay showed that the proliferation were markedly down-regulated in Flot2 silenced group compared with the control group (d0, d1, P > 0.05; d2, d3, d4, P < 0.01). E-G. Western blotting analysis of the cyclin D3, CDK2, CDK4, p27, p21 and p18 expression in Flot2 silenced HCC cells. All experiments were performed independently three times. The data were analysed by an unpaired two-tailed Student’s t-test. *indicates P < 0.05, **indicates P < 0.01.