Figure 6.

Inhibition of invasion by overexpression of GalNAc-T6. A. GALNT6 expression plasmid and control expression plasmid were transfected into HHUA cell line. Whole cell lysates (50 µg) were used for western blotting with anti-GalNAc-T6 and anti-beta actin antibodies. Exposure time of Western blot with anti-GalNAc-T6 antibody was shorter than that of Figure 3A. B. GalNAc-T6 over expressed-HHUA cell line was seeded into control and Matrigel-coated chambers at a density of 2.5 × 10⁴ cells per well. After 22 h, non-migrating and non-invading cells were removed, and migrating and invading cells were counted as described in the Materials and Methods. Cell number of each cell transfected with control plasmid was set to 1. The data were averaged from two independent experiments.