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. 2017 May 26;4(3):ENEURO.0083-17.2017. doi: 10.1523/ENEURO.0083-17.2017

Figure 5.

Figure 5.

Effects of VMAT2 deletion in VTA LepR neurons on synaptic neurotransmitter release to the amygdala. Recordings of oEPCSc and oIPSCs were made in amydalar neurons elicited by blue laser stimulation of ChR2-expressing fibers from VTA LepR neurons in brain slices. A–A’, Representative pictures showing ChR2-expressing fibers (green) in the central amygdala in low (A) and high magnification (A’). Representative recording traces of light evoked oEPSCs and oIPSCs from brain slices of control (B) and LIC::Vmat2fl°x/fl°x (KO) mice (C). Responses of oEPSCs and oIPSCs to TTX/4-AP were also shown in controls (B). D, Quantitative analyses illustrate the latency for light-evoked oIPSCs (red) and oEPSCs (green) to reach 10% peak and 100% peak of the evoked currents from the onset of laser stimulation. E, Mean amplitudes of oIPSC (red) and oEPSC (green) in controls and LIC::Vmat2fl°x/fl°x mice. Numbers in columns D, E showed frequency of successful detection of indicated currents in all recorded neurons. BLA, basolateral amygdalar nucleus; opt, optic tract. Scale bars, 100 μm (A) and 50 μm (A’). Data were presented as mean ± SEM; *p < 0.05, ***p < 0.001, unpaired Student’s t tests.