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. 2017 May 15;9(5):2077–2087.

Figure 6.

Figure 6

Changes in osteogenic protein/gene expression levels when BMSCs were treated with 10-5 M ICSII. *(P < 0.05) and **(P < 0.01) signify significantly differences between the NC and treated groups. A. BMSCs were treated with ICSII 10-5 M for 4, 6, and 8 days. Total cell extracts were prepared to assess changes in the expression of the osteogenic proteins osterix, OCN, Runx2, OPN, and bFGF by Western blot. B. BMSCs were treated with ICSII 10-5 M for 6 days, and total RNA was extracted for real-time PCR assays to detect the changes in the mRNA expression levels of the osteogenic genes osterix, OCN, Runx2, and bFGF.