Table 1.
Primers used for qPCR
| Gene | Primer sequences (5’ to 3’) | |
|---|---|---|
| TNF-α | Forward | CAAGGGACA AGGCTGCCCCG |
| Reverse | GCAGGGGCTCTTGACGGCAG | |
| IL-1β | Forward | AAGCCTCGTGCTGTCGGACC |
| Reverse | TGAGGCCCAAGGCCACAGGT | |
| IL-6 | Forward | GTACTCCAGAAGACCAGAGG |
| Reverse | TGCTGGTG ACAACCACGGCC | |
| MCP-1 | Forward | CCAGCACCAGCACCAGCCAA |
| Reverse | TGGATGCTCCAGCCGGCAAC | |
| COX2 | Forward | GATGACTGCCCAACTCCC |
| Reverse | AACCCAGGTCCTCGCTTA | |
| iNOS | Forward | CAGCTGGGCTGTACAAACCTT |
| Reverse | CATTGGAAGTGAAGCGTTTCG | |
| GAPDH | Forward | GCCAAGGCTGTGGGCAAGGT |
| Reverse | TCTCCAGGCGGCACGTCAGA | |