Figure 1.

ATRA inhibits the proliferation of rat embryo hind limb bud mesenchymal cells. A: The hind limb buds were separated from E12.5 embryos under a microscope. B: The rat hind limb bud mesenchymal cells were cultured in 60 mm plates. C: Cells were cultured in medium with 0, 0.01, 0.1, 1 and 10 μM ATRA for 24 h and using LDH cytotoxicity assay to measure cytotoxicity. D: ATRA dose-dependently inhibits proliferation of rEHBMCs in vitro. CCK-8 was used to measure cell proliferation at 6-48 h after rEHBMCs were treated with ATRA at the indicated concentrations. Data are presented as the mean ± SD of at least three independent experiments done in triplicate. *P<0.05 compared to the control.