Table 1.
PCR primer sequences for quantitative real-time PCR
| Genes | Forward primer sequence | Reverse primer sequence |
|---|---|---|
| SDF-1 | 5’-CAGGTCCGAGGAATGCTGC-3’ | 5’-CCCTGACACTGAACTGGA-3’ |
| CXCR4 | 5’-ACAGGATCTTCCTGCCCACCAT-3’ | 5’-GGGGAGTGTGATGACAAAGAGG-3’ |
| SOX9 | 5’-AACAGACGTGCAAGCTGGGAA-3’ | 5’-CGGCAGGTATTGGTCAAACTCA-3’ |
| COL2A1 | 5’-GAACTGGTGGAGCAGCAAGAGC-3’ | 5’-CCCTCAGTGGACAGTAGACGGA-3’ |
| ROCK1 | 5’-TTCATGTCCGACCTGTAACC-3’ | 5’-TTGACAGCGTTCGAGGAGAG-3’ |
| ROCK2 | 5’-TTCACGTCCGACCTGTTACC-3’ | 5’-GTGGCACCTACGGCACTCTA-3’ |
| HOXD9 | 5’-CCGCTGAAGGAGGAGGAGAAG-3’ | 5’-GCGTCTGGTATTTGGTGTAGGG-3’ |
| SHH | 5’-GGACGGCACCATTCTCATCAAC-3’ | 5’-CTCGCTTCCGCTACAGATTGC-3’ |
| BSP | 5’-GGTCAGCAAGAGCGAGTGAA-3’ | 5’-TCGAGAAAGCACTCGCCATC-3’ |
| BMP4 | 5’-GAGCCATTCCGTAGTGCCAT-3’ | 5’-AACGACCATCAGCATTCGGT-3’ |
| MLC-2 | 5’-AGCCTCAGACACCATGTCAC-3 | 5’-TGAAGCCGTCTCTGTTCTGG-3’ |
| HES-1 | 5’-CAACACGACACCGGACAAAC-3’ | 5’-TTGGAATGCCGGGAGCTATC-3’ |
| GAPDH | 5’-CAAGTTCAACGGCACAGTCAAGG-3’ | 5’-ACATACTCAGCACCAGCATCACC-3’ |