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. 2017 May 15;9(5):2327–2339.

Figure 2.

Figure 2

HPV E7 upregulated CIP2A expression via the transcription factor E2F1. A, C. Quantified real-time PCR and western blot analysis of E2F1 and CIP2A mRNA and protein levels after siRNA silencing of HPV E7 in HeLa and SiHa cells. B, D. Quantified real-time PCR and western blot analysis of CIP2A mRNA or/and protein levels after siRNA silencing or plasmid overexpression of E2F1 in HeLa and SiHa cells. For gene silencing, cells were transfected with siRNA targeting E7 (siE7), siRNA targeting E2F1 (siE2F1) or negative control siRNA (NC). For gene overexpression, cells were transfected with pCMV-E2F1 plasmid (OE-E2F1) or pCMV vector (PCMV). Data are mean SEM of triplicate experiments (*P<0.05, **P<0.01).