Figure 2.

Promotion of ROS release is involved in miR-383-induced apoptosis in ARPE-19 cells. ARPE-19 cells were transfected with control miRNA or miR-383 mimic with or without pretreatment with NAC or vehicle. ROS production and apoptosis were assessed 48 h posttransfection. A. Measurement of intracellular ROS generation using the DCFH-DA probe by flow cytometry. Top, representative flow cytometric histogram of DCF fluorescent levels. Bottom, quantification of intracellular ROS levels from three independent experiments. B. Quantification of apoptotic death by flow cytometry after Annexin-V/PI staining. *P < 0.05.