Figure 4.

Satb2 is a direct target of miR-31. A. The sequences of miR-31 and predicted binding sites in the 3’UTRs of Satb2. B. Co-transfection of hPDLSCs with the Satb2 3’UTR or Mut-Satb2 3’UTR constructs along with the miR-31 mimic or miR-control. Luciferase activities were measured (*P<0.05 compared with the cells transfected with the miR-control plus Satb2 3’UTR). C and D. The Satb2 protein expression in periodontal ligament tissues and hPDLSCs were determined by western blot. E. Western blot analysis of Satb2 expression in hPDLSCs transfected with miR-31 mimics or inhibitors or control.