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. 2017 May 15;9(5):2457–2465.

Figure 2.

Figure 2

PUMA mediates the apoptotic effects of the miR-199a-3p mimic through the mitochondrial pathway. A. HepG2 cells were transiently transfected with the miR-199a-3p mimic for 72 h. miR-199a-3p mimic mRNA content was detected by qRT-PCR assay. B. HepG2 cells were stably transfected with either a control scrambled siRNA or a PUMA siRNA and then transiently transfected with the miR-199a-3p mimic for 72 h. PUMA, active caspase-3, bcl-2, and BAX were detected by Western blot analysis. C. HepG2 cells were stably transfected with either a control scrambled siRNA or a PUMA siRNA and then transiently transfected with miR-199a-3p mimic for 72 h. Apoptosis was analyzed by annexin V/PI staining followed by flow cytometry. D. Cell viability was analyzed by LDH toxicology assay. *P<0.01 versus the control.