Figure 3.

Activation of ZHX1 mediates PUMA induction in response to miR-199a-3p mimic overexpression. HepG2 cells were stably transfected with either control siRNA or ZHX1 siRNA and then transiently transfected with the miR-199a-3p mimic for 72 h. A. ZHX1, PUMA cleaved, bcl-2, BAX, and caspase-3 protein were detected by Western blot assay. B. Apoptosis was analyzed by annexin V/PI staining followed by flow cytometry. C. Cell viability was analyzed by an LDH toxicology assay. *P<0.01 versus the control.