Expression of iNOS was down-regulated and synthesis of NO was decreased by treatment of severely burned rats with combined glutamine and probiotics. A: The relative gene expression of iNOS, eNOS and nNOS were detected for the different treatment groups by RT-PCR. B: The relative gene activities of iNOS, eNOS, and nNOS were detected for the different treatment groups. C: The expression of iNOS, eNOS, and nNOS were detected for the different treatment groups by western blot. Data are presented as the mean ± standard error of measurement (SEM) (n = 3) with *P < 0.05, **P < 0.01, or ***P < 0.001, analyzed by two-way ANOVA.