Detection and location of nerve tissues in mouse inguinal adipose tissue. (A, E, I) H&E staining of inguinal adipose tissue in neonatal mice. SC markers (green) of Sox10 (B-D), P75NTR (F-H) and MBP (J-L) were double-stained (yellow, arrow) with anti-tubulinβ3 antibody (red) in the H&E stained area. (M-O) Toluidine blue staining and TEM of inguinal adipose tissue in mice. (O) Partial enlargement of figure (N) Arrowhead: arterioles; Asterisk: small veins; Arrow: nerve tissues and SCs. Blue: DAPI. Bar = 50 μm (H&E, M), bar = 10 μm (fluorescent images, N), bar = 2 μm (O).