SEM images of M. penetrans whole cells and detergent-insoluble structures. (A) Field of M. penetrans cells. Arrows, connecting filaments. Scale bar, 1 μm. (B) Individual M. penetrans cell. Arrow, AO. Scale bar, 200 nm. (C and D) Detergent-insoluble structures from M. penetrans cells extracted with Triton X-100 (C) or Tween 20 (D). Asterisks, wide ends of structures. Scale bar, 200 nm.