FIG 5.
The ΔrseA strain exhibits membrane permeability defects that are stabilized by Mg2+ and by mutations that reduce σE activity. (A) CFU per milliliter of the ΔrseA (blue squares) and wild-type (green squares) strains from exponentially growing cultures in LB medium treated with 5% SDS (closed symbols) or 8 μg/ml rifampin (open symbols) is shown. (B) CFU per milliliter of 1-day-old stationary-phase cultures treated with 5% SDS is shown for ΔrseA cultures in LB medium (blue squares), LB-MOPS (red circles), and LB-Mg (purple circles) and wild-type cultures in LB medium (green squares). (C) CFU per milliliter of ΔrseA (blue squares), ΔrseA rpoE S172A (red circles), ΔrseA rpoE R171C (purple circles), and wild-type (green squares) stationary-phase cultures in LB medium treated with 5% SDS is shown. Data presented are averages with standard deviations from at least 2 independent cultures. In all panels, time indicates minutes after treatment.