FIG 6.
Supplementation of LB medium with Mg2+ and buffering with MOPS stabilize the ΔrseA strain in stationary phase. The ΔrseA strain was grown into stationary phase for 10 days in LB medium (blue squares), LB-Mg (purple circles), LB-MOPS (red circles), or LB-MOPS-Mg (orange circles). The wild-type strain grown in LB medium (green squares) is also shown. (A) OD600 of the cultures is shown. (B to D) CFU per milliliter is shown as total CFU per milliliter (closed symbols) and CFU per milliliter of mutants with reduced σE activity (open symbols) for cultures grown in LB-Mg (B), LB-MOPS (C), or LB-MOPS-Mg (D). Data shown are averages with standard deviations from at least 4 independent cultures.