TABLE 1.
SCAM labeling by NEM, MTSES, and MalPEG
| Residue | Blocking resulta |
Interpretation (localization) | |
|---|---|---|---|
| NEM | MTSES | ||
| Ser18 | Blocked | Unblocked | Cytoplasm |
| Ile34 | Blocked | Unblocked | Cytoplasm |
| Cys41 | Unblocked | Unblocked | Membrane |
| Ser60 | Partial blocking | Partial blocking | Periplasm (partially exposed) |
| Ser73 | Blocked | Blocked | Periplasm |
| Ser75 | Blocked | Blocked | Periplasm |
| Ile106 | Blocked | Blocked | Periplasm |
| Ala110 | Blocked | Blocked | Periplasm |
| Leu114 | Blocked | Blocked | Periplasm |
| Ser139 | Blocked | Blocked | Periplasm |
| Ala160 | Blocked | Blocked | Periplasm |
| Thr171 | Blocked | Blocked | Periplasm |
| Leu181 | Unblocked | Unblocked | Membrane |
| Ser203 | Blocked | Unblocked | Cytoplasm |
| Ser215 | Unblocked | Unblocked | Membrane |
| Gly225 | Blocked | Blocked | Periplasm |
a
“Blocked” indicates that treatment prevented MalPEG binding and that retarded gel migration was not observed. “Unblocked” indicates that treatment did not prevent MalPEG binding and that retarded gel migration was observed. “Partial blocking” indicates that treatment did not completely block the residue and that both protein species were observed.