TABLE 2.
Strains and plasmids
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| V. cholerae | ||
| O395 | Classical biotype | 56 |
| EPV102 (null) | O395 ΔvibB fbpA::cam vctP::Gentr feoABC::kan ΔviuA | 26 |
| EPV131 (null vciB::kan) | O395 ΔvibB fbpA::cam vctP::Gentr feoABC::kan ΔviuA vciB::kan | This study |
| EPV156 (null ΔvciB) | O395 ΔvibB fbpA::cam vctP::Gentr feoABC::kan ΔviuA ΔvciB | This study |
| EPV104 (vFeo) | O395 ΔvibB fbpA::cam vctP::Gentr viuA | 26 |
| EPV123 (vFeo vciB::kan) | O395 ΔvibB fbpA::cam vctP::Gentr viuA vciB::kan | This study |
| EPV153 (vFeo ΔvciB) | O395 ΔvibB fbpA::cam vctP::Gentr viuA ΔvciB | This study |
| EPV154 (vFeo ΔvciA) | O395 ΔvibB fbpA::cam vctP::Gentr viuA ΔvciA | This study |
| EPV115 (vFbp) | O395 ΔvibB ΔviuA vctP::Gentr feoABC::kan | 26 |
| EPV137 (vFbp vciB::kan) | O395 ΔvibB ΔviuA vctP::Gentr feoABC::kan vciB::kan | This study |
| EPV126 (vVib) | O395 feoABC::kan vctP::Gentr fbpA::cam | 26 |
| EPV136 (vVib vciB::kan) | O395 feoABC::kan vctP::Gentr fbpA::cam vciB::kan | This study |
| EPV103 (vVct) | O395 feoB::tmp ΔvibB fbpA::cam ΔviuA | 26 |
| EPV133 (vVct vciB::kan) | O395 feoB::tmp ΔvibB fbpA::cam ΔviuA vciB::kan | This study |
| EPV162 | O395 Δnqr | This study |
| EPV164 | O395 ΔvciB | This study |
| EPV165 | O395 ΔvciB ΔnapC | This study |
| E. coli | ||
| DH5α | Cloning strain | 57 |
| DH5α λpir | Cloning strain | 57 |
| SM10 λpir | Conjugation strain | 58 |
| Other strains | ||
| B. mallei ATCC 23344 | Source of genomic DNA | 59 |
| B. thailandensis ATCC 700388 | Burkholderia thailandensis strain used for isolating genomic DNA | 60 |
| A. hydrophila BG-2 | Aeromonas hydrophila isolate used for isolating genomic DNA | Texas Department of Health |
| Plasmids | ||
| pUC18 | Cloning vector | 61 |
| pWKS30 | Cloning vector | 62 |
| pACYC184 | Cloning vector | 63 |
| pCVD442N | Suicide vector pCVD442 with a NotI adapter inserted in the SacI site | 9 |
| pAMS23 | pHM5 carrying vciB::kan | 25 |
| pSΔvciΒ | pCVD442N carrying sequences for the deletion of the vciB locus | This study |
| pSΔvciΑ | pCVD442N carrying sequences for the deletion of the vciA locus | This study |
| pSΔnqr | pCVD442N carrying sequences for the deletion of the nqr operon | This study |
| pSΔnapC | pCVD442N carrying sequences for the deletion of napC | This study |
| pVCvciB | V. cholerae vciB cloned into pUC18 | This study |
| pBMvciB | B. mallei vciB cloned into pUC18 | This study |
| pBTvciB | B. thailandensis vciB cloned into pUC18 | This study |
| pAHvciB | A. hydrophila vciB cloned into pUC18 | This study |
| pV5vciB | V. cholerae vciB fused to an N-terminal V5 epitope tag cloned into pWKS30 | This study |
| pH8vciB | V. cholerae vciB fused to an N-terminal 8×His epitope tag cloned into pACYC184 | This study |
| pV5vciBC41S | Derivative of pV5vciB in which vciB Cys41 is mutagenized to Ser | This study |
| pV5vciB*S18C | Derivative of pV5vciBC41S with a single cysteine substitution at S18 | This study |
| pV5vciB*I34C | Derivative of pV5vciBC41S with a single cysteine substitution at N31 | This study |
| pV5vciB*S60C | Derivative of pV5vciBC41S with a single cysteine substitution at S60 | This study |
| pV5vciB*S73C | Derivative of pV5vciBC41S with a single cysteine substitution at S73 | This study |
| pV5vciB*S75C | Derivative of pV5vciBC41S with a single cysteine substitution at S89 | This study |
| pV5vciB*I106C | Derivative of pV5vciBC41S with a single cysteine substitution at I106 | This study |
| pV5vciB*A110C | Derivative of pV5vciBC41S with a single cysteine substitution at L114 | This study |
| pV5vciB*S139C | Derivative of pV5vciBC41S with a single cysteine substitution at S139 | This study |
| pV5vciB*A160C | Derivative of pV5vciBC41S with a single cysteine substitution at A160 | This study |
| pV5vciB*T171C | Derivative of pV5vciBC41S with a single cysteine substitution at T171 | This study |
| pV5vciB*L181C | Derivative of pV5vciBC41S with a single cysteine substitution at L181 | This study |
| pV5vciB*S203C | Derivative of pV5vciBC41S with a single cysteine substitution at S203 | This study |
| pV5vciB*S215C | Derivative of pV5vciBC41S with a single cysteine substitution at S215 | This study |
| pV5vciB*G225C | Derivative of pV5vciBC41S with a single cysteine substitution at G225 | This study |
| pV5vciBH38N | Derivative of pV5vciB with an H38N substitution | This study |
| pV5vciBH61N | Derivative of pV5vciB with an H61N substitution | This study |
| pV5vciBH166N | Derivative of pV5vciB with an H166N substitution | This study |
| pV5vciBW33Y | Derivative of pV5vciB with a W33Y substitution | This study |
| pV5vciBW127Y | Derivative of pV5vciB with a W127Y substitution | This study |
| pV5vciBW197Y | Derivative of pV5vciB with a W197Y substitution | This study |
| pV5vciBY142W | Derivative of pV5vciB with a Y142W substitution | This study |
| pV5vciBD180N | Derivative of pV5vciB with a D180N substitution | This study |
a
Gentr, gentamicin resistance.