FIG 6.
Glycan microarray assay of the receptor binding specificities of the H15 HA RBS mutants. The receptor binding specificity of recombinant HAs of the H15N9E190D-G225D (A) and the H15N9Q226L-G228S (B) double mutants were examined using the custom sialoside microarray. Mutations in the RBS of the H15 HA that are associated with a switch in receptor binding specificity of pandemic H1/H2/H3 viruses did not switch receptor binding specificity of the H15 HA. The mean signal and standard error were calculated from six independent replicates on the array. α2-3-Linked sialosides are shown as yellow bars (glycans 11 to 79 on the x axis), and α2-6-linked sialosides are shown as green bars (glycans 80 to 135). Glycans 1 to 10 are nonsialylated control glycans and shown in gray. Glycans imprinted on the array are listed in Table S1.