FIG 2.

pA104R binds to dsDNA with higher affinity than to ssDNA. (A and B) Reaction mixtures containing increasing concentrations of recombinant purified ASFV pA104R and 1 pmol of biotin-labeled 30-nt ssDNA (A) or biotin-labeled 30-nt dsDNA (B) were incubated for 30 min at room temperature, followed by resolution on a native polyacrylamide gel and detection using a streptavidin-HRP antibody. (C) A supershift assay was performed by adding an anti-pA104R antibody to the binding mixture 5 min after the beginning of the reaction. (D) Specific recognition of the serum used was tested against the purified pA104R by Western blotting.