FIG 1.

Inhibition of Ad infection by miR-27a/b. (A) The copy numbers of miR-27a/b in HeLa cells, H1299 cells, HUVECs, and NHLFs were determined by quantitative RT-PCR analysis. (B to D) HeLa cells were transfected with miR-27a/b mimics (B) or inhibitors (C) at the indicated doses, or H1299 cells, HUVECs, and NHLFs were transfected with miR-27a/b mimics or inhibitors at 20 nM (D). The cells were then infected with WT-Ad at 100 VP/cell. After 24 h of incubation, the copy numbers of WT-Ad genomic DNA in the cells were determined by quantitative PCR analysis. (E) HeLa cells were transfected with miR-27a/b mimics or inhibitors at 20 nM, followed by infection with WT-Ad at 100 VP/cell. After 24 h of incubation, IFU titers of the WT-Ad progeny in the cells were determined by infectious titer assay. (F) HeLa cells were transfected with a control plasmid (pHM5-U6) or a pre-miR-27a/b-expressing plasmid (pHM5-U6-pre-miR-27a or -b), followed by infection with WT-Ad at 100 VP/cell. After 24 h of incubation, the copy numbers of WT-Ad genomic DNA in the cells were determined. The data are expressed as means and SD (n = 3 or 4). *, P < 0.05; **, P < 0.01; ***, P < 0.001.