FIG 5.

Reduction in Ad replication via cell cycle arrest by knockdown of TXN. (A) HeLa cells (2 × 10⁵ cells/well) were transfected with siTXN2 at 50 nM. At the indicated days posttransfection, the cell numbers were counted. (B and C) HeLa-FUCCI cells were transfected with miR-27a/b mimics (B) and siTXN2 (C) at 20 nM and 50 nM, respectively. After 48 h of incubation, fluorescence photomicrographs of the cells were obtained. Cells in the G₀-G₁, S, and G₂-M phases show red, yellow, and green fluorescence, respectively. Representative images from multiple experiments are shown. Percentages of cells in each phase are shown in the bar graphs. (D) HeLa cells were pretreated with PD0332991 at 1 μM, followed by infection with WT-Ad at 100 VP/cell. After 24 h of incubation, the copy numbers of WT-Ad genomic DNA in the cells were determined by quantitative PCR analysis. DMSO, dimethyl sulfoxide. (E) HeLa cells were transfected with siTXN2 at 50 nM, followed by infection with WT-Ad at 100 VP/cell. After 3 h of incubation, the copy numbers of WT-Ad genomic DNA in the cells were determined. The data are expressed as means and SD (n = 3 or 4). ***, P < 0.001; n.s., not significant.