FIG 6.

Restoration of miR-27a/b-mediated inhibition of Ad infection by overexpression of VA-RNAs. (A) HeLa cells were transfected with a control plasmid (ΔNaeI; pAdVAntage-ΔNaeI) or a VA-RNA-expressing plasmid (pAdVAntage). After 48 h of incubation, the copy numbers of miR-27a/b in the cells were determined by quantitative RT-PCR analysis. (B and C) HeLa cells were transfected with a pre-miR-27a- or -b-expressing plasmid (pHM5-U6-pre-miR-27a or -b) (B) or with siRNA at 50 nM (C), followed by infection with Sub720 at 100 VP/cell. After 24 h of incubation, the copy numbers of Sub720 genomic DNA were determined by quantitative PCR analysis. (D) HeLa cells were cotransfected with pHM5-U6-pre-miR-27a or -b and pAdVAntage, followed by infection with Sub720 at 100 VP/cell. After 24 h of incubation, the copy numbers of Sub720 genomic DNA in the cells were determined. The data are expressed as means and SD (n = 4). *, P < 0.05; **, P < 0.01.