TABLE 2.
Oligonucleotides and primers used in this study
| Oligonucleotide no. | Name | Sequence (5′–3′)a |
|---|---|---|
| 1 | pre-miR-27a-S | CACCAGGGCTTAGCTGCTTGTGAGCAGGGTCCACACCAAGTCGTGTTCACAGTGGCTAAGTTCCGCTTTTT |
| 2 | pre-miR-27a-AS | GCATAAAAAGCGGAACTTAGCCACTGTGAACACGACTTGGTGTGGACCCTGCTCACAAGCAGCTAAGCCCT |
| 3 | pre-miR-27b-S | CACCAGAGCTTAGCTGATTGGTGAACAGTGATTGGTTTCCGCTTTGTTCACAGTGGCTAAGTTCTGCTTTTT |
| 4 | pre-miR-27b-AS | GCATAAAAAGCAGAACTTAGCCACTGTGAACAAAGCGGAAACCAATCACTGTTCACCAATCAGCTAAGCTCT |
| 5 | Ad5-F | GGGATCGTCTACCTCCTTTTGA |
| 6 | Ad5-R | GGGCAGCAGCGGATGAT |
| 7 | Ad5-probe | FAM-ACAGAAACCCGCGCTACCATACTGGAG-TAMRA |
| 8 | GAPDH-F | GGTGGTCTCCTCTGACTTCAACA |
| 9 | GAPDH-R | GTGGTCGTTGAGGGCAATG |
| 10 | GAPDH-probe | FAM-CACTCCTCCACCTTTGACGCTGGG-TAMRA |
| 11 | ts1-F | GAACCACCTACCCTTCACG |
| 12 | ts1-R | CCGCCAACATTACAGACTCG |
| 13 | RPS6KA5-F | CTCCTCACTGTCAAGCACGAG |
| 14 | RPS6KA5-R | GCCTTTTGAACGATTGTTGCCT |
| 15 | ANKRD40-F | GCGGCCTTAGGGGACATTC |
| 16 | ANKRD40-R | GTCCAGCCGTTGACCTCATT |
| 17 | GOLM1-F | TGGCCTGCATCATCGTCTTG |
| 18 | GOLM1-R | CCCTGGAACTCGTTCTTCTTCA |
| 19 | PRKX-F | GCAGGACTTTGACACGCTG |
| 20 | PRKX-R | GGCGAAGAAATGCTTGGCTG |
| 21 | GABRP-F | CTTGGCCTTCGTGTGTCTGAG |
| 22 | GABRP-R | CCGACCTCGACGTTGAACT |
| 23 | ATP6V1A-F | GGGTGCAGCCATGTATGAG |
| 24 | ATP6V1A-R | TGCGAAGTACAGGATCTCCAA |
| 25 | CSRP2-F | TGGGAGGACCGTGTACCAC |
| 26 | CSRP2-R | CCGTAGCCTTTTGGCCCATA |
| 27 | PDS5B-F | GATGTTCGCTTACTGGTAGCC |
| 28 | PDS5B-R | TCTAGCCCCTTCAACTGTCTT |
| 29 | GPAM-F | GATGTAAGCACACAAGTGAGGA |
| 30 | GPAM-R | TCCGACTCATTAGGCTTTCTTTC |
| 31 | SNAP25-F | ACCAGTTGGCTGATGAGTCG |
| 32 | SNAP25-R | CAAAGTCCTGATACCAGCATCTT |
| 33 | DCUN1D4-F | GCCGCCGCTGTCAATTTTC |
| 34 | DCUN1D4-R | AGGTTCAGCTTATTAAGGGTGTG |
| 35 | TXN2-F | CTGGTGGCCTGACTGTAACAC |
| 36 | TXN2-R | TGACCACTCGGTCTTGAAAGT |
| 37 | NR2F6-F | GAGCGGCAAGCATTACGGT |
| 38 | NR2F6-R | GGCAGGTGTAGCTGAGGTT |
| 39 | SEC62-F | CCAGCAGAAATGAGAGTAGGTG |
| 40 | SEC62-R | GAGTCAATGAAGCCCACATCA |
| 41 | CDH11-F | AGAGAGCCCAGTACACGTTGA |
| 42 | CDH11-R | TTGGCATGATAGGTCTCGTGC |
| 43 | ADORA2B-F | TGCACTGACTTCTACGGCTG |
| 44 | ADORA2B-R | GGTCCCCGTGACCAAACTT |
| 45 | TMUB1-F | GCAGCTACCGACAGCATGAG |
| 46 | TMUB1-R | ACCTGCTCTGAATCATTGAGGA |
| 47 | PDK4-F | GGAGCATTTCTCGCGCTACA |
| 48 | PDK4-R | ACAGGCAATTCTTGTCGCAAA |
| 49 | SLC7A11-F | TCTCCAAAGGAGGTTACCTGC |
| 50 | SLC7A11-R | AGACTCCCCTCAGTAAAGTGAC |
| 51 | TMBIM6-F | CATATAACCCCGTCAACGCAG |
| 52 | TMBIM6-R | GCAGCCGCCACAAACATAC |
| 53 | MBTD1-F | GGCATGGCTACCTGTGAGATG |
| 54 | MBTD1-R | GGCCAAAATGCTTGCCTTCT |
| 55 | PAPSS2-F | AGACGGAGAACCAGCAGAAAT |
| 56 | PAPSS2-R | CACACGGTACATCCTCGGAAC |
| 57 | SFXN2-F | GTCAATATCCCCATGATGCGAC |
| 58 | SFXN2-R | AGCTCTCCGGGAATGACCAA |
| 59 | TNRC18-F | TCTCTGTCGCTGAGTAACGTC |
| 60 | TNRC18-R | CCCCGTCACCATGAGGTTG |
| 61 | TXN2-3′UTR-F | CGACCTCGAGATCAGAGGATGGTGGTGCTG |
| 62 | TXN2-3′UTR-R | TTGCGGCCGCGGAGGCACCTTGAGACTTCC |
| 63 | SNAP25-3′UTRmut1-F | CCACAGTATTGTTCTTGTAAAAAGCTTACATTCCACAGAGTTACTGCCACGG |
| 64 | SNAP25-3′UTRmut1-R | CCGTGGCAGTAACTCTGTGGAATGTAAGCTTTTTACAAGAACAATACTGTGG |
| 65 | SNAP25-3′UTRmut2-F | GGAGAGAGCAATCTTGCGGATCCAACAGTGTGGATGTAAATTTTATAAGGC |
| 66 | SNAP25-3′UTRmut2-R | GCCTTATAAAATTTACATCCACACTGTTGGATCCGCAAGATTGCTCTCTCC |
| 67 | SNAP25-3′UTRmut3-F | GAATGAAATATAAAAGCTTAGATAAATATCATTATAGCATGTAATATTAAATTCCTCC |
| 68 | SNAP25-3′UTRmut3-R | GGAGGAATTTAATATTACATGCTATAATGATATTTATCTAAGCTTTTATATTTCATTC |
| 69 | TXN2-3′UTRmut1-F | CTCATTTTTTGTAGGTCACTAGTAAAGAACTTAGTCATCCCTTCCACCTC |
| 70 | TXN2-3′UTRmut1-R | GAGGTGGAAGGGATGACTAAGTTCTTTACTAGTGACCTACAAAAAATGAG |
| 71 | TXN2-3′UTRmut2-F | CATCCCTTCCACCTCCTAGTCTAGAGAACAGACCCTGGGTCC |
| 72 | TXN2-3′UTRmut2-R | GGACCCAGGGTCTGTTCTCTAGACTAGGAGGTGGAAGGGATG |
| 73 | TXN2-cloning-F | GGACCCAGGGTCTGTTCTCTAGACTAGGAGGTGGAAGGGATG |
| 74 | TXN2-cloning-R | GGACCCAGGGTCTGTTCTCTAGACTAGGAGGTGGAAGGGATG |
a
FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.