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ICP0 is required for the removal of Nectin-1 from the surface of the infected cells. The ICP0-null mutant or the wild-type virus was used to infect HEp-2 cells (5 PFU/cell) seeded in F150 flasks, as described above. The cells were harvested at 6 h postinfection. Detection of the surface Nectin-1 was done as described in the legend of Fig. 2.
